Agrobacterium-mediated Transformation and Transgenic Plant Regeneration from Embryogenic Calli Derived from an Immature Seed Produced from Miniature Rose Cultivar 'Shortcake'
نویسندگان
چکیده
منابع مشابه
Development of Transgenic Tall Fescue Plants from Mature Seed-derived Callus via Agrobacterium-mediated Transformation
We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing β-g...
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The purpose of the research study is to select the best rice explant and variety for Agrobacterium mediated genetic transformation studies. In vitro callus induction and regeneration frequency of two explants i.e. immature embryo and mature grains of 9 rice varieties (Oryza sativa L.) was investigated by culturing explants source on N6 media supplemented with 2, 4-D at 2mg/l for callus inductio...
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The present study establishes a regeneration protocol and optimizes conditions for Agrobacterium-mediated transformation of the tetraploid emmer wheat, Triticum dicoccum. Regeneration from mature and immature embryos was accomplished as a two-step process involving callus induction in the presence of 2,4-D followed by regeneration on a 2,4-D free, cytokinin-containing medium (RM1). Higher conce...
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Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
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Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2003
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.20.291